Should you encounter any unexpected behaviour,
please let us know.

* AF-1F0N_recycle_12 *

B-factor analysis for ID 2402070206021787280

Overall B-factor prediction

Correlation= -0.626 for 284 C-alpha atoms.

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

B-factors are computed based on the first 100 normal modes (B-PREDICT) and are scaled to match the overall B-factors in the submitted model (B-SCALED). A high correlation with the observations is an indicator that the normal modes capture well the overall flexibility features of the model. Comparison of the observed (B-OBS, blue) and the computed (B-SCALED, red) B-factors for all C-alpha atoms is presented below. Differences between B-factors from normal mode analysis and from X-ray crystallographic data may for example indicate regions where the protein flexibility is modified due to external factors such as tight crystall-packing.

ATOM TYP RES RESID B-PREDICT B-SCALED B-OBS	B-SCALED	B-OBS
2 CA SER 1 0.0267 98.1641 83.8800
8 CA ARG 2 0.0560 104.9228 85.3800
19 CA PRO 3 0.1529 127.3431 84.8100
26 CA GLY 4 0.1256 121.0289 87.1900
30 CA LEU 5 0.0261 98.0128 92.9400
38 CA PRO 6 0.0285 98.5756 94.2500
45 CA VAL 7 0.0133 95.0586 97.1200
52 CA GLU 8 0.0139 95.2019 97.5600
61 CA TYR 9 0.0118 94.7162 97.1900
73 CA LEU 10 0.0123 94.8351 98.3800
81 CA GLN 11 0.0160 95.6893 98.3100
90 CA VAL 12 0.0117 94.6836 98.6200
97 CA PRO 13 0.0168 95.8647 98.5000
104 CA SER 14 0.0138 95.1710 98.5600
110 CA PRO 15 0.0291 98.7098 98.3800
117 CA SER 16 0.0197 96.5455 98.0600
123 CA MET 17 0.0152 95.4901 98.1900
131 CA GLY 18 0.0301 98.9493 98.0600
135 CA ARG 19 0.0213 96.9049 97.7500
146 CA ASP 20 0.0202 96.6557 98.3100
154 CA ILE 21 0.0094 94.1618 98.5000
162 CA LYS 22 0.0088 94.0256 98.6200
171 CA VAL 23 0.0064 93.4659 98.7500
178 CA GLN 24 0.0062 93.4177 98.6200
187 CA PHE 25 0.0077 93.7594 98.6200
198 CA GLN 26 0.0069 93.5707 98.1900
207 CA SER 27 0.0104 94.3901 97.7500
213 CA GLY 28 0.0124 94.8582 97.1200
217 CA GLY 29 0.0390 100.9971 96.1900
221 CA ASN 30 0.0528 104.1886 95.9400
229 CA ASN 31 0.0333 99.6917 96.3100
237 CA SER 32 0.0179 96.1231 97.8800
243 CA PRO 33 0.0086 93.9626 98.0600
250 CA ALA 34 0.0067 93.5244 98.6200
255 CA VAL 35 0.0039 92.8845 98.8800
262 CA TYR 36 0.0040 92.9065 98.8800
274 CA LEU 37 0.0032 92.7361 98.8100
282 CA LEU 38 0.0033 92.7555 98.6900
290 CA ASP 39 0.0051 93.1724 97.6900
298 CA GLY 40 0.0055 93.2545 95.8800
302 CA LEU 41 0.0126 94.8873 94.0000
310 CA ARG 42 0.0171 95.9443 94.8800
321 CA ALA 43 0.0115 94.6453 96.1900
326 CA GLN 44 0.0147 95.3841 94.5600
335 CA ASP 45 0.0247 97.7069 94.1200
343 CA ASP 46 0.0264 98.0886 95.1900
351 CA TYR 47 0.0119 94.7318 96.8800
363 CA ASN 48 0.0071 93.6339 98.0600
371 CA GLY 49 0.0082 93.8773 97.7500
375 CA TRP 50 0.0063 93.4493 98.3100
389 CA ASP 51 0.0093 94.1423 97.1900
397 CA ILE 52 0.0124 94.8412 96.5600
405 CA ASN 53 0.0132 95.0400 96.7500
413 CA THR 54 0.0109 94.5128 96.8800
420 CA PRO 55 0.0129 94.9656 96.5000
427 CA ALA 56 0.0088 94.0205 97.5000
432 CA PHE 57 0.0083 93.9133 98.0000
443 CA GLU 58 0.0128 94.9467 97.2500
452 CA TRP 59 0.0100 94.3017 97.1200
466 CA TYR 60 0.0088 94.0105 97.5000
478 CA TYR 61 0.0172 95.9728 96.6200
490 CA GLN 62 0.0373 100.6152 95.1200
499 CA SER 63 0.0159 95.6543 97.1900
505 CA GLY 64 0.0153 95.5250 97.3800
509 CA LEU 65 0.0076 93.7532 98.5000
517 CA SER 66 0.0073 93.6678 98.6200
523 CA ILE 67 0.0045 93.0260 98.7500
531 CA VAL 68 0.0044 92.9988 98.8100
538 CA MET 69 0.0039 92.8828 98.6900
546 CA PRO 70 0.0043 92.9749 98.4400
553 CA VAL 71 0.0070 93.5958 98.3100
560 CA GLY 72 0.0077 93.7627 96.4400
564 CA GLY 73 0.0073 93.6633 95.3100
568 CA GLN 74 0.0079 93.8201 97.1200
577 CA SER 75 0.0067 93.5430 96.5000
583 CA SER 76 0.0051 93.1592 97.3100
589 CA PHE 77 0.0037 92.8459 98.0000
600 CA TYR 78 0.0038 92.8523 98.4400
612 CA SER 79 0.0060 93.3838 98.3100
618 CA ASP 80 0.0068 93.5505 98.5000
626 CA TRP 81 0.0097 94.2382 98.5000
640 CA TYR 82 0.0147 95.3884 98.1200
652 CA SER 83 0.0229 97.2809 97.9400
658 CA PRO 84 0.0195 96.5003 97.7500
665 CA ALA 85 0.0139 95.2028 97.4400
670 CA CYS 86 0.0235 97.4219 97.3100
676 CA GLY 87 0.0397 101.1606 95.6200
680 CA LYS 88 0.0765 109.6757 94.6200
689 CA ALA 89 0.0786 110.1479 92.1200
694 CA GLY 90 0.0840 111.4049 95.1200
698 CA CYS 91 0.0334 99.7067 96.1200
704 CA GLN 92 0.0218 97.0342 95.3100
713 CA THR 93 0.0127 94.9126 97.9400
720 CA TYR 94 0.0087 93.9894 98.1900
732 CA LYS 95 0.0071 93.6180 98.6200
741 CA TRP 96 0.0060 93.3686 98.6900
755 CA GLU 97 0.0064 93.4691 98.6900
764 CA THR 98 0.0075 93.7140 98.7500
771 CA PHE 99 0.0061 93.3958 98.8800
782 CA LEU 100 0.0060 93.3697 98.7500
790 CA THR 101 0.0084 93.9197 98.6900
797 CA SER 102 0.0103 94.3728 98.5000
803 CA GLU 103 0.0091 94.0834 98.6900
812 CA LEU 104 0.0077 93.7662 98.8100
820 CA PRO 105 0.0140 95.2222 98.5600
827 CA GLN 106 0.0200 96.6088 98.3800
836 CA TRP 107 0.0143 95.2865 98.6200
850 CA LEU 108 0.0106 94.4408 98.6900
858 CA SER 109 0.0183 96.2186 98.3100
864 CA ALA 110 0.0310 99.1584 98.0000
869 CA ASN 111 0.0229 97.2714 98.3100
877 CA ARG 112 0.0188 96.3281 97.9400
888 CA ALA 113 0.0232 97.3538 96.9400
893 CA VAL 114 0.0136 95.1218 98.1900
900 CA LYS 115 0.0131 95.0093 95.8100
909 CA PRO 116 0.0135 95.1130 96.8800
916 CA THR 117 0.0193 96.4492 97.4400
923 CA GLY 118 0.0132 95.0431 97.7500
927 CA SER 119 0.0060 93.3763 98.2500
933 CA ALA 120 0.0056 93.2751 98.8100
938 CA ALA 121 0.0037 92.8486 98.8800
943 CA ILE 122 0.0036 92.8140 98.8100
951 CA GLY 123 0.0040 92.9083 98.5600
955 CA LEU 124 0.0040 92.9036 97.7500
963 CA SER 125 0.0045 93.0285 97.0600
969 CA MET 126 0.0041 92.9266 96.2500
977 CA ALA 127 0.0038 92.8613 98.0600
982 CA GLY 128 0.0037 92.8514 98.2500
986 CA SER 129 0.0038 92.8547 97.9400
992 CA SER 130 0.0036 92.8157 98.5600
998 CA ALA 131 0.0042 92.9629 98.8100
1003 CA MET 132 0.0038 92.8567 98.7500
1011 CA ILE 133 0.0053 93.2108 98.8100
1019 CA LEU 134 0.0053 93.2066 98.8800
1027 CA ALA 135 0.0071 93.6218 98.7500
1032 CA ALA 136 0.0079 93.8039 98.7500
1037 CA TYR 137 0.0086 93.9700 98.7500
1049 CA HIS 138 0.0101 94.3158 98.6900
1059 CA PRO 139 0.0161 95.7062 98.3800
1066 CA GLN 140 0.0260 98.0053 98.1200
1075 CA GLN 141 0.0076 93.7353 98.5600
1084 CA PHE 142 0.0066 93.5171 98.7500
1095 CA ILE 143 0.0070 93.5954 98.7500
1103 CA TYR 144 0.0060 93.3630 98.8100
1115 CA ALA 145 0.0045 93.0301 98.8800
1120 CA GLY 146 0.0043 92.9857 98.8100
1124 CA SER 147 0.0039 92.8881 98.7500
1130 CA LEU 148 0.0037 92.8358 98.6200
1138 CA SER 149 0.0044 93.0120 97.8100
1144 CA ALA 150 0.0050 93.1384 97.6200
1149 CA LEU 151 0.0063 93.4372 97.3800
1157 CA LEU 152 0.0054 93.2300 98.1200
1165 CA ASP 153 0.0084 93.9198 97.0600
1173 CA PRO 154 0.0091 94.0959 96.3800
1180 CA SER 155 0.0129 94.9569 96.8800
1186 CA GLN 156 0.0209 96.8239 94.3100
1195 CA GLY 157 0.0919 113.2339 89.1200
1199 CA MET 158 0.0399 101.1986 88.4400
1207 CA GLY 159 0.0170 95.9055 92.3800
1211 CA PRO 160 0.0174 96.0142 95.0600
1218 CA SER 161 0.0322 99.4278 94.0600
1224 CA LEU 162 0.0279 98.4293 91.3800
1232 CA ILE 163 0.0152 95.4959 95.8100
1240 CA GLY 164 0.0182 96.1973 96.3800
1244 CA LEU 165 0.0474 102.9414 94.1900
1252 CA ALA 166 0.0300 98.9128 93.8100
1257 CA MET 167 0.0149 95.4306 96.7500
1265 CA GLY 168 0.0241 97.5472 96.0600
1269 CA ASP 169 0.0405 101.3549 93.8800
1277 CA ALA 170 0.0260 97.9903 93.3100
1282 CA GLY 171 0.0241 97.5606 93.4400
1286 CA GLY 172 0.0264 98.0938 96.8800
1290 CA TYR 173 0.0161 95.7132 97.6900
1302 CA LYS 174 0.0186 96.2814 97.5000
1311 CA ALA 175 0.0122 94.8034 97.1900
1316 CA ALA 176 0.0159 95.6626 97.1200
1321 CA ASP 177 0.0138 95.1649 98.0600
1329 CA MET 178 0.0089 94.0314 98.3800
1337 CA TRP 179 0.0105 94.4025 98.3100
1351 CA GLY 180 0.0177 96.0695 97.7500
1355 CA PRO 181 0.0421 101.7292 96.7500
1362 CA SER 182 0.0361 100.3411 95.3800
1368 CA SER 183 0.0640 106.7801 95.5600
1374 CA ASP 184 0.0334 99.7186 97.8800
1382 CA PRO 185 0.0302 98.9640 97.6900
1389 CA ALA 186 0.0159 95.6697 98.0600
1394 CA TRP 187 0.0094 94.1667 98.3800
1408 CA GLU 188 0.0127 94.9292 97.9400
1417 CA ARG 189 0.0083 93.8970 98.1200
1428 CA ASN 190 0.0071 93.6220 98.3800
1436 CA ASP 191 0.0068 93.5606 98.3800
1444 CA PRO 192 0.0063 93.4501 98.6200
1451 CA THR 193 0.0096 94.2055 98.5000
1458 CA GLN 194 0.0102 94.3381 98.4400
1467 CA GLN 195 0.0086 93.9791 98.6900
1476 CA ILE 196 0.0108 94.4715 98.8100
1484 CA PRO 197 0.0183 96.2187 98.6900
1491 CA LYS 198 0.0155 95.5773 98.7500
1500 CA LEU 199 0.0103 94.3708 98.8100
1508 CA VAL 200 0.0146 95.3571 98.7500
1515 CA ALA 201 0.0269 98.2094 98.6900
1520 CA ASN 202 0.0205 96.7307 98.5000
1528 CA ASN 203 0.0198 96.5658 98.5000
1536 CA THR 204 0.0118 94.7100 98.6900
1543 CA ARG 205 0.0088 94.0217 98.8100
1554 CA LEU 206 0.0075 93.7306 98.8800
1562 CA TRP 207 0.0057 93.2963 98.8100
1576 CA VAL 208 0.0055 93.2571 98.8100
1583 CA TYR 209 0.0059 93.3534 98.6200
1595 CA CYS 210 0.0054 93.2278 97.8800
1601 CA GLY 211 0.0093 94.1454 96.2500
1605 CA ASN 212 0.0138 95.1728 91.3800
1613 CA GLY 213 0.0166 95.8134 89.0600
1617 CA THR 214 0.0237 97.4758 89.0000
1624 CA PRO 215 0.0286 98.5888 78.2500
1631 CA ASN 216 0.0400 101.2345 84.7500
1639 CA GLU 217 0.0928 113.4485 78.8100
1648 CA LEU 218 0.0534 104.3410 69.7500
1656 CA GLY 219 0.0333 99.6808 83.8100
1660 CA GLY 220 0.0259 97.9671 78.4400
1664 CA ALA 221 0.0593 105.6841 79.1200
1669 CA ASN 222 0.0618 106.2651 85.8100
1677 CA ILE 223 0.0838 111.3586 80.8800
1685 CA PRO 224 0.0702 108.2246 84.1900
1692 CA ALA 225 0.0272 98.2780 83.9400
1697 CA GLU 226 0.0229 97.2906 89.7500
1706 CA PHE 227 0.0309 99.1206 86.0600
1717 CA LEU 228 0.0208 96.7881 88.8800
1725 CA GLU 229 0.0103 94.3749 92.1900
1734 CA ASN 230 0.0144 95.3189 91.0000
1742 CA PHE 231 0.0157 95.6152 89.6900
1753 CA VAL 232 0.0102 94.3463 94.7500
1760 CA ARG 233 0.0103 94.3695 95.8800
1771 CA SER 234 0.0140 95.2300 93.5600
1777 CA SER 235 0.0087 94.0070 96.3800
1783 CA ASN 236 0.0071 93.6362 98.3100
1791 CA LEU 237 0.0126 94.8992 98.0000
1799 CA LYS 238 0.0158 95.6369 97.5000
1808 CA PHE 239 0.0091 94.0832 98.6200
1819 CA GLN 240 0.0099 94.2821 98.6900
1828 CA ASP 241 0.0252 97.8072 98.5600
1836 CA ALA 242 0.0210 96.8461 98.6200
1841 CA TYR 243 0.0132 95.0325 98.8100
1853 CA ASN 244 0.0217 97.0107 98.6200
1861 CA ALA 245 0.0685 107.8142 98.5600
1866 CA ALA 246 0.0412 101.5012 98.5600
1871 CA GLY 247 0.0519 103.9845 98.4400
1875 CA GLY 248 0.0237 97.4742 98.5600
1879 CA HIS 249 0.0328 99.5596 98.1200
1889 CA ASN 250 0.0196 96.5265 98.5000
1897 CA ALA 251 0.0124 94.8584 98.6900
1902 CA VAL 252 0.0110 94.5321 98.3800
1909 CA PHE 253 0.0083 93.9001 98.5600
1920 CA ASN 254 0.0091 94.0796 98.2500
1928 CA PHE 255 0.0109 94.5049 97.8100
1939 CA PRO 256 0.0170 95.9055 96.0600
1946 CA PRO 257 0.0406 101.3753 93.6900
1953 CA ASN 258 0.0218 97.0146 93.6900
1961 CA GLY 259 0.0122 94.7955 94.1200
1965 CA THR 260 0.0110 94.5334 95.3100
1972 CA HIS 261 0.0087 94.0081 95.1900
1982 CA SER 262 0.0111 94.5430 95.5000
1988 CA TRP 263 0.0090 94.0675 95.7500
2002 CA GLU 264 0.0123 94.8254 94.9400
2011 CA TYR 265 0.0081 93.8594 97.1200
2023 CA TRP 266 0.0074 93.6983 97.9400
2037 CA GLY 267 0.0091 94.0983 97.8100
2041 CA ALA 268 0.0097 94.2346 97.6200
2046 CA GLN 269 0.0066 93.5208 98.4400
2055 CA LEU 270 0.0065 93.4884 98.4400
2063 CA ASN 271 0.0111 94.5578 97.8100
2071 CA ALA 272 0.0124 94.8437 98.1200
2076 CA MET 273 0.0095 94.1830 97.9400
2084 CA LYS 274 0.0093 94.1239 98.2500
2093 CA GLY 275 0.0179 96.1195 98.0600
2097 CA ASP 276 0.0130 94.9902 98.4400
2105 CA LEU 277 0.0099 94.2791 98.5600
2113 CA GLN 278 0.0135 95.1023 98.0600
2122 CA SER 279 0.0256 97.8936 97.7500
2128 CA SER 280 0.0190 96.3699 97.6900
2134 CA LEU 281 0.0141 95.2523 97.8800
2142 CA GLY 282 0.0248 97.7240 94.8100
2146 CA ALA 283 0.0268 98.1733 89.4400
2151 CA GLY 284 0.0968 114.3628 69.2500

If you find results from this site helpful for your research, please cite one of our papers:

K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004.
K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography.

elNémo is maintained by Yves-Henri Sanejouand.
It was developed by Karsten Suhre.
Between 2003 and 2014, it was hosted by IGS (Marseille).
Last modification: April 25th, 2023.

Should you encounter any unexpected behaviour, please let us know.